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    xmlns:ns0="http://metadb.riken.jp/db/bioresource_schema/"
    xmlns:ns5="http://metadb.riken.jp/terms/xsearch#"
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:ns9="http://purl.obolibrary.org/obo/"
    xmlns:skos="http://www.w3.org/2004/02/skos/core#"
    xmlns:ns7="http://semanticscience.org/resource/"
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  <rdfs:Class rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC00158">
    <ns0:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_8"/>
    <ns0:brs_geneticBackground rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/BackgroundStrain_32"/>
    <ns5:resourceTypeLink rdf:resource="http://metadb.riken.jp/terms/xsearch#resource_type_transgene"/>
    <ns11:citesAsAuthority rdf:resource="http://rdf.ncbi.nlm.nih.gov/pubmed/10529728"/>
    <rdfs:label>C57BL/6-Tg(gpt delta)1Nmi/Rbrc</rdfs:label>
    <ns0:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns0:brs_hasGenomicFeature>
    <rdfs:seeAlso rdf:resource="http://www2.brc.riken.jp/lab/animal/detail.php?brc_no=RBRC00158"/>
    <ns0:brs_hasAllele rdf:resource="http://metadb.riken.jp/db/mgi_rdf/MGI:3612991"/>
    <ns0:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns0:brs_hasGenomicFeature>
    <ns0:brs_strainTypeLink rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/straintype_2"/>
    <ns1:references rdf:parseType="Resource">
    </ns1:references>
    <ns1:isRecombinant rdf:datatype="http://www.w3.org/2001/XMLSchema#boolean"
    >true</ns1:isRecombinant>
    <ns0:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_4"/>
    <ns1:colonyMaintenance xml:lang="ja">兄妹交配　ホモ型トランスジェニックとして固定。</ns1:colonyMaintenance>
    <ns1:strainDevelopment xml:lang="en">This strain was developed for efficient detection of mutations in vivo by Dr. Nohmi T.</ns1:strainDevelopment>
    <ns5:addinfo>Necessary documents for ordering:&lt;ol&gt;&lt;li&gt;Order form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Category I MTA: MTA for distribution with RIKEN BRC (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Acceptance of responsibility for living modified organism (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;/ol&gt;</ns5:addinfo>
    <ns0:brs_geneticBackground rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/geneticBackground_100"/>
    <ns6:organism rdf:parseType="Resource">
    </ns6:organism>
    <ns1:donorDNA>lambda phage red/gam, P1 phage loxP sites, E. coli gpt delta, E. coli chloramphenicol acetyltransferase (CAT) gene, chiC</ns1:donorDNA>
    <ns11:citesAsAuthority rdf:resource="http://rdf.ncbi.nlm.nih.gov/pubmed/10725358"/>
    <ns1:expectedDelivery xml:lang="ja">C（3〜6か月）</ns1:expectedDelivery>
    <ns11:citesAsAuthority rdf:resource="http://rdf.ncbi.nlm.nih.gov/pubmed/8991079"/>
    <ns0:brs_preservationStatus rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainStatus_3"/>
    <ns1:strainDevelopment xml:lang="en">This strain was developed for efficient detection of mutations in vivo by Dr.Nohmi T.</ns1:strainDevelopment>
    <ns11:citesAsAuthority rdf:resource="http://rdf.ncbi.nlm.nih.gov/pubmed/11358805"/>
    <ns0:brs_strainTypeLink rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainType_24"/>
    <ns1:references rdf:parseType="Resource">
    </ns1:references>
    <dcterms:references rdf:resource="http://identifiers.org/pubmed/10503911"/>
    <dc:description xml:lang="en">This strain is useful to detect point mutations and deletions with high efficiency and sensitivity in vivo.  The mice carry lambda EG10 DNA as a transgene. When the rescued phages are infected into Escherichia coli YG6020-expressing Cre recombinase, the phage DNA is converted into plasmid pYG142 carrying the chloramphenicol-resistance gene and the gpt gene of E. coli. The gpt mutants can be positively detected as colonies arising on plates containing chloramphenicol and 6-thioguanine. The EG10 DNA carries a chi site along with the red and gam genes so that the wild-type phages display Spi-(sensitive to P2 interference) phenotype. Mutant phages lacking both red and gam genes can be positively detected as plaques that grow in P2 lysogens of E. coli.</dc:description>
    <dcterms:references rdf:resource="http://identifiers.org/pubmed/10462718"/>
    <skos:altLabel xml:lang="ja">C57BL/6-TgN(gpt Delta)</skos:altLabel>
    <skos:altLabel xml:lang="en">C57BL/6-TgN(gpt Delta)</skos:altLabel>
    <rdfs:subClassOf rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/Strain"/>
    <dcterms:references rdf:resource="http://identifiers.org/pubmed/8991079"/>
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    </ns0:brs_hasAppearance>
    <ns11:citesAsAuthority rdf:resource="http://rdf.ncbi.nlm.nih.gov/pubmed/10503911"/>
    <ns11:citesAsAuthority rdf:resource="http://rdf.ncbi.nlm.nih.gov/pubmed/11062167"/>
    <ns9:RO_0002162 rdf:resource="http://purl.obolibrary.org/obo/NCBITaxon_10090"/>
    <ns5:addinfo>&lt;a href='https://brc.riken.jp/mus/pcr00158'&gt;Genotyping protocol -PCR-&lt;/a&gt;</ns5:addinfo>
    <rdfs:label xml:lang="en">C57BL/6-TgN(gpt Delta)</rdfs:label>
    <ns1:references rdf:parseType="Resource">
    </ns1:references>
    <ns1:strainDevelopment xml:lang="ja">国立食品衛生安全性研究所の能美博士により生体を用いた突然変異検出系として開発。</ns1:strainDevelopment>
    <ns1:references rdf:parseType="Resource">
    </ns1:references>
    <ns1:references rdf:parseType="Resource">
    </ns1:references>
    <ns0:brs_depositedBy rdf:parseType="Resource">
    </ns0:brs_depositedBy>
    <ns0:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns0:brs_hasGenomicFeature>
    <ns11:citesAsAuthority rdf:resource="http://rdf.ncbi.nlm.nih.gov/pubmed/10462718"/>
    <ns0:brs_preservationStatus rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainStatus_2"/>
    <ns7:SIO_001279 rdf:parseType="Resource">
    </ns7:SIO_001279>
    <ns0:brs_depositorOrganization xml:lang="en">Natl. Inst. Health Sci.</ns0:brs_depositorOrganization>
    <dcterms:references rdf:resource="http://identifiers.org/pubmed/10462717"/>
    <dcterms:references rdf:resource="http://identifiers.org/pubmed/11358805"/>
    <ns0:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns0:brs_hasGenomicFeature>
    <ns1:references rdf:parseType="Resource">
    </ns1:references>
    <dcterms:references rdf:resource="http://identifiers.org/pubmed/10725358"/>
    <ns0:brs_depositor xml:lang="en">Takehiko NOUMI</ns0:brs_depositor>
    <ns0:brs_depositor xml:lang="ja">能美　健彦</ns0:brs_depositor>
    <dc:description xml:lang="ja">点突然変異と欠失突然変異の検出および解析のために開発されたトランスジェニマウス</dc:description>
    <ns0:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns0:brs_hasGenomicFeature>
    <ns0:brs_depositorOrganization xml:lang="ja">国立医薬品食品衛生研究所</ns0:brs_depositorOrganization>
    <dcterms:references rdf:resource="http://identifiers.org/pubmed/10529728"/>
    <ns1:references rdf:parseType="Resource">
    </ns1:references>
    <ns1:expectedDelivery xml:lang="en">C (3-6 months)</ns1:expectedDelivery>
    <ns5:termsAndConditions xml:lang="ja">条件を付加しない。</ns5:termsAndConditions>
    <ns1:references rdf:parseType="Resource">
    </ns1:references>
    <ns0:brs_hasGenomicFeature rdf:parseType="Resource">
    </ns0:brs_hasGenomicFeature>
    <ns5:termsAndConditions xml:lang="en">No specific terms and conditions.    (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)</ns5:termsAndConditions>
    <ns1:references rdf:parseType="Resource">
    </ns1:references>
    <dcterms:references rdf:resource="http://identifiers.org/pubmed/11062167"/>
    <dcterms:identifier>RBRC00158</dcterms:identifier>
    <ns11:citesAsAuthority rdf:resource="http://rdf.ncbi.nlm.nih.gov/pubmed/10462717"/>
  </rdfs:Class>
  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/Phenotype_1012881">
    <ns0:brs_indicatesStrain rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC00158"/>
  </rdf:Description>
  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/RecoveryTestResult_49">
    <ns0:brs_indicatesStrain rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC00158"/>
  </rdf:Description>
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